Sateesh Kagale

 

Roles and responsibilities

  • Research Scientist, Crop Genetics and Genomics
  • Team Leader, Advanced Data Analytics
  • Adjunct Professor, Department of Plant Sciences, University of Saskatchewan

Current research and/or projects

Current research focus is on application of genome analysis and editing technologies for crop improvement.

Research and/or project statements

  • Canola genetic enhancement: optimization of canola seed quality traits, including the quantity, quality and processability of seed protein, oil and fibre.
  • Meiosis and recombination: developing advanced genetic and genomic resources to enhance meiotic recombination to create novel gene combinations for crop breeding.
  • Single cell sequencing in plants: microspore-based single cell sequencing and genotyping strategy for monitoring the frequency of meiotic recombination in plants.
  • Gene editing platform: breeder-friendly gene editing platform to improve agronomics and seed quality traits of crop varieties.
  • Advancing domestication: harnessing the precision of gene editing in crop breeding

Education

  • Ph.D. Biology, Western University (formerly University of Western Ontario), Canada
  • M.Sc. Plant Pathology, Tamil Nadu Agricultural University, India
  • B.Sc. Agriculture, University of Agricultural Sciences Dharwad, India

Affiliations

  • Canadian Society of Plant Biologists
  • SynBio Canada

Awards

  • Outstanding Achievement in Science Award, Agriculture and Agri-Food Canada (2017)
  • Award of Recognition for Leadership Excellence, Aquatic and Crop Resource development Research Centre (2016) 
  • Gold Harvest Award, Agriculture and Agri-Food Canada (2015)
  • NSERC Visiting Fellowship (2008-2010) 
  • Ontario Graduate Scholarship, Ministry of Training, Colleges and Universities (2005-2006) 
  • President’s Scholarship for Graduate Studies, University Western Ontario (2002-2003)
  • Gold Medalist, Tamil Nadu Agricultural University (2002)
  • Gold Medalist, University of Agricultural Sciences  Dharwad (1999)

Key publications

  • Lyzenga, W.J., Pozniak, C.J., and Kagale, S. (2021). Advanced domestication: harnessing the precision of gene editing in crop breeding. Plant Biotechnology Journal 19, 660-670.
  • Lyzenga, W.J., and Kagale, S. (2021). Wheat improvement using genome editing technology. BioTechniques 71:577-579.
  • Gao, P., Quilichini, T.D., Yang, H., Li, Q., Nilsen, K.T., Qin, L., Babic, V., Liu, L., Cram, D., Pasha, A., Esteban, E., Condie, J., Sidebottom, C., Zhang, Y., Huang, Y., Zhang, W., Bhowmik, P., Kochian, L.V., Konkin, D., Wei, Y., Provart, N.J., Kagale, S., Smith, M., Patterson, N., Gillmor, C.S., Datla, R., and Xiang, D. (2021). Evolutionary divergence in embryo and seed coat development of U’s Triangle Brassica species illustrated by a spatiotemporal transcriptome atlas. New Phytologist 233:30-51.
  • Kagale, S. and Close T.J. (2021) Legume: Embracing the Genome Era. Legume Science 3: e113.
  • Bhowmik, P., Konkin, D., Polowick, P., Hodgins, C.L., Subedi, M., Xiang, D., Yu, B., Patterson, N., Rajagopalan, N., Babic, V., Ro, D.-K., Tar'an, B., Bandara, M., Smyth, S.J., Cui, Y., and Kagale, S. (2021). CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges. Legume Science 3: e96.
  • Moghaddam, S.M., Oladzad, A., Koh, C., Ramsay, L., Hart, J.P., Mamidi, S., Hoopes, G., Sreedasyam, A., Wiersma, A., Zhao, D., Grimwood, J., Hamilton, J.P., Jenkins, J., Vaillancourt, B., Wood, J.C., Schmutz, J., Kagale, S., Porch, T., Bett, K.E., Buell, C.R., and McClean, P.E. (2021). The tepary bean genome provides insight into evolution and domestication under heat stress. Nature communications 12, 2638.
  • Walkowiak, S., Gao, L., Monat, C., Haberer, G., Kassa, M.T..…Kagale, S…..Pozniak, C.J. (2020). Multiple wheat genomes reveal global variation in modern breeding. Nature. 588, 277-283.
  • Perumal, S., Koh, C.S., Jin, L., Buchwaldt, M., Higgins, E.E., Zheng, C., Sankoff, D., Robinson, S.J., Kagale, S., Navabi, Z.-K., Tang, L., Horner, K.N., He, Z., Bancroft, I., Chalhoub, B., Sharpe, A.G., and Parkin, I.A.P. (2020). A high-contiguity Brassica nigra genome localizes active centromeres and defines the ancestral Brassica genome. Nature Plants 6, 929-941.
  • Cram, D., Kulkarni, M., Buchwaldt, M., Rajagopalan, N., Bhowmik, P., Rozwadowski, K., Parkin, I.A.P., Sharpe, A.G., and Kagale, S. (2020). WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat. BMC Plant Biology 19, 474.
  • Huang D., Zheng Q., Melchkart T., Bekkaoui Y., Konkin D.J.F., Kagale S., Martucci M., You FM., Clarke M., Adamski N.M., Chinoy C., Steed A., McCartney C., Cutler A.J., Nicholson P., Feurtado J.A. (2020). Dominant Inhibition of Awn Development by a Zinc-Finger Transcriptional Repressor Expressed at the B1 Locus in Wheat. New Phytologist 225: 340-355.
  • Shunmugam, A.S.K., Bollina, V., Dukowic-Schulze, S., Bhowmik, P.K., Ambrose, C., Higgins, J.D., Pozniak, C., Sharpe, A.G., Rozwadowski, K., and Kagale, S. (2018). MeioCapture: an efficient method for staging and isolation of meiocytes in the prophase I sub-stages of meiosis in wheat. BMC plant biology, 18: 293.
  • Bhowmik, P., Ellison, E., Polley, B., Bollina, V., Kulkarni, M., Ghanbarnia, K., Song, H., Gao, C., Voytas, D.F., and Kagale, S. (2018). Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Scientific reports, 8: 6502.
  • Kulkarni, M., Soolanayakanahally, R., Ogawa, S., Uga, Y., Selvaraj, M.G., and Kagale, S. (2017). Drought response in wheat: key genes and regulatory mechanisms controlling root system architecture and transpiration efficiency. Frontiers in Chemistry, 5: 106.
  • Kagale, S., Nixon, J., Khedikar, Y., Pasha, A., Provart, N.J., Clarke, W.E., Bollina, V., Robinson, S.J., Coutu, C., Hegedus, D.D., Sharpe, A.G., and Parkin, I.A. (2016). The developmental transcriptome atlas of the biofuel crop Camelina sativa. Plant journal, 88: 879-894.
  • Kagale, S., Robinson, S.J., Nixon, J., Xiao, R., Huebert, T., Condie, J., Kessler, D., Clarke, W.E., Edger, P.P., Links, M.G., Sharpe, A.G., and Parkin, I.A. (2014). Polyploid evolution of the Brassicaceae during the Cenozoic era. Plant cell, 26: 2777-2791.
  • Kagale, S., Koh, C., Nixon, J., Bollina, V., Clarke, W.E., Tuteja, R., Spillane, C., Robinson, S.J., Links, M.G., Clarke, C., Higgins, E.E., Huebert, T., Sharpe, A.G., and Parkin, I.A. (2014). The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure. Nature communications, 5: 3706.
  • Parkin, I.A., Koh, C., Tang, H., Robinson, S.J., Kagale, S., Clarke, W.E., Town, C.D., Nixon, J., Krishnakumar, V., Bidwell, S.L., Denoeud, F., Belcram, H., Links, M.G., Just, J., Clarke, C., Bender, T., Huebert, T., Mason, A.S., Pires, J.C., Barker, G., Moore, J., Walley, P.G., Manoli, S., Batley, J., Edwards, D., Nelson, M.N., Wang, X., Paterson, A.H., King, G., Bancroft, I., Chalhoub, B., and Sharpe, A.G. (2014). Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea. Genome Biology, 15: R77.
  • Borg, M., Rutley, N., Kagale, S., Hamamura, Y., Gherghinoiu, M., Kumar, S., Sari, U., Esparza-Franco, M.A., Sakamoto, W., Rozwadowski, K., Higashiyama, T., and Twell, D. (2014). An EAR-Dependent Regulatory Module Promotes Male Germ Cell Division and Sperm Fertility in Arabidopsis. Plant cell, 26: 2098-2113.
  • Kagale, S., and Rozwadowski, K. (2011). EAR motif-mediated transcriptional repression in plants: an underlying mechanism for epigenetic regulation of gene expression. Epigenetics, 6: 141-146.
  • Kagale, S., Links, M.G., and Rozwadowski, K. (2010). Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in Arabidopsis. Plant physiology, 152: 1109-1134.
Sateesh Kagale

Sateesh Kagale

Associate Research Officer
Aquatic and Crop Resource Development
110 Gymnasium Place
Saskatoon, Saskatchewan S7N 0W9
Preferred language: English
Telephone: 306-975-4194

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Expertise

Agriculture, Genomics