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NRC PowerBlade - Transcript
[0:00:00] Music plays
[0:00:00] Video shows a view of the Powerblade, a portable centrifuge instrument for running tests on microfluidic lab on a chip devices. We see the inside of the PowerBlade start to spin.
[On screen text] PowerBlade
[On screen text] The next generation centrifugal microfluidic platform, enabling advanced pneumatic control during high speed rotation
[0:00:19] Video zooms in to the top part of the PowerBlade to focus on a lab on a chip device.
[On screen text] Simple manifold interface, Microfluidic cartridge, Fast temperature cycling zones, 8 pneumatic lines per cartridge, integrated air pump, valves and pressure sensor
[On screen graphic] "Centrifugal force" is written inside an arrow pointing outwards from the centre of the device.
[0:00:33] Video shows a lab on a chip device is detached from the PowerBlade and a close-up of the chip moves into the foreground.
[On screen text] Pressure ports, Channels (<30 to 500 microliters), Reagent reservoirs, Injected thermoplastics
[On screen text] Simple cartridges; Low cost, No active element, Mass manufacturing with thermoplastics, No surface treatment, Tolerant to manufacturing errors, Negligible dead volume, Wide range of volume (microliters to millilitres), Integration of complex protocols
[0:00:55] Video shows a lab on a chip device reattaching itself to the PowerBlade.
[On screen text] The combination of centrifugal and pneumatic forces enables advanced fluidic operations
[On screen graphic] "Centrifugal force" is written inside an arrow pointing outwards from the centre of the device.
[0:01:04] Video shows PowerBlade starts spinning, while the focus is kept on the lab on a chip device.
[On screen text] Sedimentation and blood fractionation
[0:01:08] Video shows liquid being added to one of the lab on a chip's reservoirs and air escaping from the PowerBlade's tubes.
[On screen text] Negative air pressure
[On screen text] Transfer of supernatant to waste reservoir
[0:01:10] Video shows air entering from the PowerBlade's tubes and liquid transferring from the one of the chip's reservoirs to another.
[On screen text] Positive air pressure
[On screen text] On-demand transfer of reagents
[0:01:22] Video shows air entering from the PowerBlade's tubes and bubbling occurring in one of the chip's reservoirs.
[On screen text] Positive air pressure
[On screen text] Bubble mixing and bead resuspension
[0:01:22] Video shows air entering and leaving from the PowerBlade's tubes and various mixing, heating and cooling operations occurring on the chip.
[On screen text] Transfer of supernatant to waste reservoir
[On screen text] Negative air pressure
[On screen text] Positive air pressure
[On screen text] Programmable on-chip dispensing
[On screen text] Positive air pressure
[On screen text] Bubble mixing and bead resuspension
[On screen text] Sedimentation and blood fractionation
[On screen text] Accurate and fast temperature cycling
[0:01:53] Video zooms out to a full view of the Powerblade.
[On screen text] Highly customizable, Fast assay development, High rotation speed (>1000 rpm), Professional control software, Integrated computer, Automated operation
[On screen text] PowerBlade, Enabling deployment of complex bio-analytical assays
[0:02:14] Video fade to black
[0:02:18] Music stops
[On screen graphic] Official signature, National Research Council Canada / Conseil national de recherches Canada
[On screen graphic] Government of Canada wordmark