Mammalian expression systems employing Chinese hamster ovary (CHO) cells are the workhorse of the biopharma industry, and were used to produce five of the ten top-selling biologics in 2016: Humira, Rituximab, Avastin, Herceptin, and EnbrelFootnote 1. CHO cells have also become the expression system of choice in an R&D setting, enabling the rapid, large-scale production of hundreds of protein variants for various in vitro and in vivo applications.
Over the past 20 years, the NRC has made crucial improvements to its pTT® vectors, cell lines, transfection vehicle and culture medium that comprise its proprietary CHO expression platform. These improvements in both stable and transient systems enable the fast, high-yield, cost-effective production of most protein modalities. Our proprietary cumate switch enables users to turn the expression of a given gene on and off during production, a complementary feature that can be useful for production of cytostatic/toxic proteins, or for cell engineering.
- Non-exclusive R&D and/or commercial exploitation licence
- R&D agreement for development of custom cell lines
- Transient expression of recombinant proteins for R&D purposes
- Fully-scalable stable expression of recombinant proteins and lead candidates for R&D and commercial purposes
How it works
Transient expression: CHO-3E7
A stable CHO clone expressing a truncated but functional form of EBNA1 (CHO-3E7) was obtained and shown to enhance recombinant protein production when using pTT® vectors. The use of an optimized cytomegalovirus (CMV)-based expression cassette on the oriP-bearing pTT® vector also increased productivity significantly. Further enhancement was achieved by co-expressing protein kinase B (Akt) and adding valproic acid followed by a temperature shift.
Stable expression: CHOBRI TM
This platform uses the cumate-inducible expression platform to generate CHO pools that stably express between 200 and 2000 mg/L in under four weeks post-transfection: two weeks for pool selection and expansion, and two for production. The pools can be maintained for months in culture under selection pressure while maintaining expression levels. Frozen/thawed pools also retain their productivity.
The use of a Scaffold/Matrix Attachment Region (S/MAR) sequence in the expression vector helps increase the level of expression and prevents silencing of the transgene expression cassette.
The CHO pools can be used to isolate stable clones that express 1-4 g/L of monoclonal antibodies and other recombinant proteins. The clones are stable for over 100 generations and are amenable to GMP manufacturing. There are currently several CHOBRI TM cell lines under GMP manufacturing to support clinical trials.
- High yield, low cost protein production for research and biomanufacturing
- Transient or stable expression
- Family of validated pTT® vectors
- Choice of proprietary cell lines or custom cell line development to best suit your expression needs
- Cumate switch allows you to turn the expression of a given gene on and off during production
- Serum-free media enables easy recovery of recombinant proteins
- Compatible with commercially available feeds; custom fed batch development
- CHOBRI is a trademark of the National Research Council of Canada.
- pTT® is a registered trademark of the National Research Council of Canada.
- NRC file 11266 (pTT® vector/cassette): Patents granted in the United States, Canada, Europe, and Singapore.
- NRC file 11992 (CHO-3E7 cell line): Patents granted in the United States and Europe, pending in Canada.
- NRC file 12671 (CHOBRI TM cell line): Transferred as a trade secret.
- NRC file 12273 (S/MAR sequence to increase stability): Patents granted in the United States, Europe, China, and Singapore; pending in Canada and South Korea.
- NRC file 11225/11648 (Cumate switch): Patents granted in Canada, the United States, and Europe.